Program
Organizers: Herb Boyer, Stu Linn, Mila Pollock, and Rich Roberts
Session 1: The Beginnings of the Field
Chairperson: Stu Linn, University of California, Berkeley, Berkeley, CA
- Tom Bickle
- Variations on a theme: the families of restriction/modification enzymes
- Werner Arber
- Microbial Genetics is at the origin of molecular genetics
- Ham Smith
- Discovery of the first Type II restriction enzyme and its aftermath
- Mila Pollock
- Illuminating the history of science, the importance of historical archives
- Bruno Strasser
- Restriction enzymes: between nature, culture and politics
Session 2: The Restriction Enzyme Field Begins to Grow
Chairperson: David Dryden, University of Edinburgh, Edinburgh, UK
- Matt Meselson
- The discovery of EcoKI
- Herb Boyer
- The discovery of EcoRI and its uses in recombinant DNA
- Clyde Hutchison
- Restriction enzymes and DNA sequencing
- Ken Horiuchi
- Work in Norton Zinder’s lab and Japan
Session 3: Restriction Enzymes Become Commercial Reagents
Chairperson: Srinivasan Chandrasegaran, Johns Hopkins University, Baltimore, MD
- Rich Roberts
- Many more REs at CSHL, the start of REBASE and more recent work
Conversations: Commercialization
- Ira Schildkraut
- First commercial sales and the start of NEB
- Jack Chirikjian
- Starting Bethesda Research Laboratories
- Bill Linton
- The early days of Promega
- Arvydas Janulaitis
- Science and politics: three phases of commercialization at Fermentas
- Panel Discussion
Session 4: Cloning and Sequencing of RM Systems
Chairperson: Ham Smith, J. Craig Venter Institute, La Jolla, CA
- Geoff Wilson
- The cloning efforts at NEB
- Arvydas Lubys
- The cloning efforts at Fermentas
- Rick Morgan
- The MmeI family and engineering opportunities
Session 5: The Biochemistry of Restriction Enzymes
Chairperson: V. Nagaraja, Indian Institute of Science, Bangalore, India
- Steve Halford
- Type II restriction enzymes: searching for one site and then two
- Alfred Pingoud
- Sequence specific recognition and engineering
- Andrzej Piekarowicz
- H. influenzae and N. gonorrhoeae RM systems and their biological implications
Session 6: Structural Studies
Chairperson: Herb Boyer, University of California, San Francisco, San Francisco, CA
- John Rosenberg
- EcoRI structure
- Aneel Aggarwal
- The current state of structural studies
- Xiaodong Cheng
- Methylase and RE structures
Session 7: Short Contributions and Summary
Chairperson: Rich Roberts, New England BioLabs, Ipswich, MA
Short solicited contributions
- Robert Yuan
- University of Maryland, College Park, MD
- Lise Raleigh
- New England BioLabs, Ipswich, MA
- David Dryden
- University of Edinburgh, Edinburgh, UK
- Ichizo Kobayashi
- University of Tokyo, Tokyo, Japan
- Other contributors:
Bill Studier
,
Gary Wilson
,
Paul Roy
Summarization and Close of Meeting
- Stu Linn
- University of California, Berkeley, Berkeley, CA
